New stability indicating RP-HPLC method was developed and validated for the estimation of cinacalcet hydrochloride in tablet dosage form. The separation was achieved on C18, (250 mm x 4.6 mm x 5 µm) column using a mobile phase composition of 0.2% triethyl amine in water (pH-6.5 with OPA) and methanol (20:80% V/V). Eluents were detected at 225 nm at 1 ml/min flow rate. Stress studies were performed with milder conditions followed by stronger conditions so as to get sufficient degradation around 20%. A total of five degradation products were detected and separated from analyte. The linearity of the proposed method was investigated in the range of 40 - 160 µg/ml for cinacalcet. The limit of detection and limit of quantification was found to be 0.89 µg/ml and 2.69 µg/ml respectively. Precision % RSD was found to be less than 2% and the recovery was between 99-101%.
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